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. 2013 May 19;104(8):1097–1106. doi: 10.1111/cas.12181

Figure 7.

Figure 7

Comprehensive search for Helios target genes by microarray analysis. (a,b) Gene expression analysis of Jurkat stable cells. The gene expression patterns of Jurkat cells expressing Hel‐5 (n = 3), shIkaros (n = 3), and shHelios (n = 3) were comprehensively analyzed by microarray technique. The obtained 2D hierarchical clusters and Pearson's correlation between the cells expressing Hel‐5 or not (a) and the cells introducing shHel, shIk, or shSc (b). (c) Venn diagram of differential gene expression pattern in the Jurkat sublines. The each differential expression gene set (5‐fold changes, P < 1 × 10−5) was compared. (d) Venn diagram depicting the overlap between the outputs of pathway analysis in Jurkat sublines. The analysis was based on the NCI‐Nature Pathway Interaction Database.37 Each differential pathway set (t‐test, P < 0.01) was compared and the common pathways listed. (e) Results of quantitative RT‐PCR of shingosine‐1‐phosphate receptor 1 (S1PR1) and receptor 3 (S1PR3) in Jurkat sublines (n = 3, mean ± SD). HDAC, histone deacetylase; VEGFR, vascular endothelial growth factor receptor.