Figure 7.

Reduced Mmp9 expression in tumors derived from AX cells overexpressing Twist2. (a) Expression of Twist2 and Fbln5 in GFP‐positive cells isolated from tumors formed in syngeneic mice at 7 days after subcutaneous injection of AX‐Mock or AX‐Twist2 cells. (b) Mmp9 expression in total tumor tissue formed as in (a). (c) The expression of FBLN5 and MMP9 in human osteosarcoma (OS) specimens and bone marrow stromal cells (BMSC) was evaluated using microarray analysis. The ratio of the abundance of each mRNA to that of β‐actin (ACTB) mRNA is presented for each specimen as well as in box‐and‐whisker plots. *P < 0.05. ***P < 0.001.