Figure 1.

Resveratrol activates ataxia–telangiectasia mutated kinase (ATM) signaling in leukemia cells. (a) Leukemia cells were treated with resveratrol or vehicle for 6 h then stained with an antibody specific for phosphorylated ATM (at Ser188) and analyzed with flow cytometry. (b) Leukemia cells were treated for 24 h with the indicated dose of resveratrol and the expression of activated Chk2 in the cellular lysates was assessed with Western blotting. Representative results using Molt4 and OUN1 cells are shown in panels (a and b). (c) Leukemia cells were treated with resveratrol for 12 h and a cell cycle analysis was carried out using flow cytometry. Representative results of three independent experiments are shown.