Figure 5.

Resveratrol modulates the expression of NKG2D receptor in natural killer (NK) cells. (a) NK cells from healthy donors were cultured in the presence or absence of resveratrol for 48 h and their expression of NKG2D receptors were assessed with flow cytometry. A representative result is shown in the left panel and summarized data from four donors are shown in the right panel. (b) NK cells were treated as in panel (a) and their expression of NKG2D receptors was examined with Western blotting using anti‐NKG2D mAb (clone 149810). The same blots were stripped and reprobed with anti‐GAPDH antibodies equal protein loading. A representative result of three independent experiments is shown. Densitometric analysis of the bands obtained in three independent experiments (mean ± SEM) is shown in the right panel. (c) NK cells were treated with resveratrol for 24 h and the levels of NKG2D transcripts were assessed using RT‐PCR. Expression levels were normalized to GAPDH. Data represent mean ± SEM from four donors are shown.