Figure 6.

Resveratrol modulates the expression of protein kinases associated with natural killer (NK) cell activation. (a) NK cells were treated with the indicated concentrations of resveratrol for 24 h and their expressions of phosphorylated ERK1/2, JNK1/2, and signal transducer and activator of transcription‐3 (STAT3) were examined with Western blotting. Representative results of three independent experiments are shown. Natural killer cells were cultured for 24 h in the presence or absence of resveratrol. The cells were harvested and cultured for 48 h in plates coated with human IgG or a mixture of five recombinant human NKG2D ligands. The levels of tumor necrosis factor‐α (TNF‐α) (b) and Granzyme B (c) released into the culture medium were measured using ELISA. Mean ± SEM of three independent experiments is shown.