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. 2013 Feb 14;104(3):391–397. doi: 10.1111/cas.12076

Figure 4.

Figure 4

The effect of ASCL2 on resistance to 5‐fluorouracil. (A) MKN‐1 cells were seeded in 96‐well plates, and the cell growth of MKN‐1 cells were analyzed by CCK‐8 reagent after 24 h exposure to the indicated doses of 5‐fluorouracil. (B) Cell growth of MKN‐1 cells were measured at 24, 48 and 72 h after exposure to 5‐fluorouracil (10 μg/mL). (C) Differences (ASCL2 divided by Vector) in cell growth in panel B. (D) A plot of caspase‐3/7 activity normalized to protein concentration in MKN‐1 cells after 48 h exposure to 5‐fluorouracil (10 μg/mL). Columns, results of triplicate experiments; error bars, SD. *P < 0.05, **P < 0.01, ***P < 0.001.