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. 2013 Jan 30;104(3):313–320. doi: 10.1111/cas.12087

Figure 5.

Figure 5

Restoration of interleukin (IL)‐2 cannot rescue MAGI‐1 expression in transformed cells. (A) Two distinct Tax1‐transformed IL‐2‐independent CTLL‐2 cells (clone 1, 3) were cultured with or without IL‐2 for 1 week, and the expression of MAGI‐1, Tax1 and Tubulin in the transformed cells with IL‐2 (lane 3, 5), the transformed cells without IL‐2 (lane 2, 4) and parental CTLL‐2 cells with IL‐2 (lane 1) were measured by a Western blotting analysis. (B) hAkt1 mΔPH‐transformed IL‐2‐independent CTLL‐2 cells were cultured with or without IL‐2 for 1 week, and the expressions of MAGI‐1, Akt1 and Tubulin proteins in the hAkt1 mΔPH‐expressing cells with IL‐2 (lane 2), the hAkt1 mΔPH‐transformed cells without IL‐2 (lane 3), the hAkt1 mΔPH‐transformed cells with IL‐2 (lane 4) as well as the vector‐transduced CTLL‐2 cells cultured in the presence of IL‐2 (lane 1) were measured by a Western blotting analysis.