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. 2020 Nov 5;80(3):396–409.e6. doi: 10.1016/j.molcel.2020.10.005

Figure 2.

Figure 2

Memory of Prior IFNγ Induction Is Reversible but Persists for up to 14 Days in Continuously Cycling Cells

(A) Scheme outlining the experiment to determine the duration of IFNγ-mediated transcriptional memory.

(B) Cells subjected to the IFNγ treatment regimen outlined in (A) were processed for western blotting and probed for GBP5 protein expression. α-TUB, loading control.

(C) Cells as in (B) but processed for RT-qPCR of GBP5 and HLA-DRA mRNA. Expression levels were normalized to ACTB and internally to cells after priming. Error bars, SD; n = 3 biological replicates; numbers represent the p values.