Figure 4.

GBP5 and HLA-DRA mRNA Levels in Primed Cells Return to the Pre-induced Levels of Naive Cells, and Ongoing Transcription Is Not Required for Maintenance of Transcriptional Memory

(A) Scheme to measure long-term transcriptional output of memory genes following priming.

(B) HeLa cells were subjected to the IFNγ treatment regimen outlined in (A) and processed for RT-qPCR of GBP5 and HLA-DRA mRNA. Signals were normalized to ACTB expression and internally to naive cells. Error bars, SD; n = 3 biological replicates; numbers represent the p values.

(C) Representation of processed RNA-seq data in HeLa cells analogous to data in Figure 1C but for primed over naive cells.

(D) Outline of the triptolide-based RNA Pol II inhibition experiment.

(E) HeLa cells were subjected to the IFNγ and triptolide treatment regimen outlined in (D) and processed for RT-qPCR of GBP5 mRNA. Signals were normalized to ACTB expression. Error bars, SD; n = 3 biological replicates.

See also Figure S3.