REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
GBP5	Cell Signaling Technology	Cat#67798; RRID: AB_2799735
GBP5	Abcam	Cat#ab96119; RRID: AB_10678091
IRF1	Cell Signaling Technology	Cat#8478; RRID: AB_10949108
EGFP	Chromotek	Cat#3H9; RRID: AB_10773374
α-TUB	Merck	Cat#T9026; RRID: AB_477593
Anti-rabbit (fluorophore conjugated)	LI-COR	Cat#926-32211; RRID: AB_621843
Anti-mouse (fluorophore conjugated)	Rockland	Cat#610-744-124; RRID: AB_1057600
RNA Pol II	Abcam	Cat#ab817; RRID: AB_306327
HA	Merck	Cat#12CA5; RRID: AB_514505
H3K27ac	Abcam	Cat#ab4729; RRID: AB_2118291
H3K36me3	Abcam	Cat#ab9050; RRID: AB_306966
H3K79me2	Abcam	Cat#ab3594; RRID: AB_303937
H3K4me2	Abcam	Cat#ab32356; RRID: AB_732924
SCC1 (RAD21)	Abcam	Cat#ab992; RRID: AB_2176601
Bacterial and Virus Strains
lentiCRISPR v2	Sanjana et al., 2014; Addgene	Addgene #52961
lentiCRISPR v2-Blast	Addgene	Addgene #83480
psPAX2	Addgene	Addgene #12260
pMD2.G	Addgene	Addgene #12259
Chemicals, Peptides, and Recombinant Proteins
Interferon gamma (IFNγ)	Merck	Cat#SRP3058; CAS: 9008-11-1
Triptolide	Merck	Cat#T3652; CAS: 38748-32-2
Indole-3-acetic acid sodium salt (Auxin, IAA)	Merck	Cat#I5148; CAS: 6505-45-9
Critical Commercial Assays
NEBNext Ultra RNA Library Prep Kit for Illumina	New England Biolabs	Cat#E7530
NEBNext Poly(A) mRNA Magnetic Isolation Module	New England Biolabs	Cat#E7490
NEBNext Ultra II Directional RNA Library Prep Kit	New England Biolabs	Cat#E7760
Nextera XT DNA Library Preparation Kit	Illumina	Cat#FC-131-10
TDE1 Tagment DNA Enzyme	Illumina	Cat#15027865
Deposited Data
Raw and analyzed data, ATAC-seq	this paper	GEO: GSE150199
Raw and analyzed data, ChIP-seq	this paper	GEO: GSE150199
Raw and analyzed data, RNA-seq	this paper	GEO: GSE150199
Raw and analyzed data, RNA-seq_SCC1-AID	this paper	GEO: GSE150199
Raw and analyzed data, single-cell RNA-seq	this paper	GEO: GSE150199
Unprocessed western blots	this paper; Mendeley Data	http://dx.doi.org/10.17632/86yrzx7sfb.2
Experimental Models: Cell Lines
Human: HeLa WT	ATCC	Cat#CCL-2; RRID: CVCL_0030
Human: Hs27 (male primary fibroblasts)	Jonathan Howard lab, Instituto Gulbenkian de Ciência, Portugal	RRID: CVCL_0335
Human: HeLa EGFP::GBP5	this paper	N/A
Human: HeLa H3.3-HA	Bodor et al., 2013	N/A
Human: HeLa Kyoto SCC1-EGFP-AID osTIR1+	Wutz et al., 2017	N/A
Human: HeLa Kyoto SCC1-EGFP-AID osTIR1-	Wutz et al., 2017	N/A
Human: HeLa Kyoto	https://web.expasy.org/cellosaurus/CVCL_1922	RRID: CVCL_1922
Human: HeLa Kyoto site A cohesin mutant polyclonal population 1	this paper	N/A
Human: HeLa Kyoto site A cohesin mutant polyclonal population 2	this paper	N/A
Human: HeLa Kyoto site B cohesin mutant polyclonal population	this paper	N/A
Human: HeLa Kyoto site C cohesin mutant polyclonal population	this paper	N/A
Human: HeLa Kyoto site C cohesin mutant clonal population	this paper	N/A
Human: HCT116	ATCC	CCL-247; RRID: CVCL_0291
Human: hTERT RPE-1	ATCC	CRL-4000; RRID: CVCL_4388
Human: HEK293	ATCC	CRL-1573; RRID: CVCL_0045
Oligonucleotides
RT-qPCR primers (see Table S4)	this paper	N/A
Mutagenesis oligonucleotides (see Table S4)	this paper	N/A
Recombinant DNA
pX330-U6-Chimeric_BB-CBh-hSpCas9	Cong et al., 2013; Addgene	Addgene #42230
EGFP-FLAG-miniAID-FLAG synthetic construct	this paper	N/A
Software and Algorithms
GPP sgRNA Designer software	Doench et al., 2016	https://portals.broadinstitute.org/gpp/public/analysis-tools/sgrna-design
Bwa-mem version 0.7.17.1	Li, 2013	http://bio-bwa.sourceforge.net/
Htseq-count version 0.9.1	Anders et al., 2015	https://htseq.readthedocs.io/en/master/count.html
DESeq2 version 2.11.40.6	Love et al., 2014	http://bioconductor.org/packages/release/bioc/html/DESeq2.html
Bowtie2 version 2.3.4.3	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
bamCompare	Ramírez et al., 2016	https://deeptools.readthedocs.io/en/develop/content/tools/bamCompare.html