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. 2020 Nov 2;133(20):jcs248823. doi: 10.1242/jcs.248823

Fig. 3.

Fig. 3.

CD98hc HH domain mediates the binding between β1 integrin and TRPV4. (A) Diagram of TRPV4 constructs showing transmembrane, proline-rich (PR), and ankyrin repeat (AR, blue) domains, as well as myc (yellow) tags. (B) Immunoblots showing myc-tagged TRPV4 constructs immunoprecipitated with β1 integrin (left) or CD98hc (middle) antibody in lysates from HEK293T cells transfected with the indicated DNA constructs. (C) Immunoblots showing β1 integrin and TRPV4 immunoprecipitated with β1 integrin and TRPV4 antibodies, respectively, in lysates from HEK293T cells transfected with increasing amounts (2.5, 5 µg) of the CD98hc(HH) domain construct. (D) Histogram showing the densitometric quantification of immunoblot signals from experiments described in C. White bars, vector control; grey bars, CD98hc(HH) low; black bars, CD98hc(HH) high (n=3, all data shown are mean±s.e.m. *P<0.05).