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(A) Design and fabrication of the microfluidic device used for 3D cell spheroid culture. The two-layered device is made of gas permeable PDMS. The top layer is designed for fluid flow and the bottom channel is exploited for cell entrapment and subsequent formation of 3D cell spheroids. (B) Experimental photos of the fabricated device. The inset shows a brightfield microscopic image of the cell culture chambers in the bottom layer. Scale bar is 200 μm.
