Fig 4. VEGF-A concentration measured from the conditioned medium collected from the monolayer and 3D spheroid cell cultures, and the calculated VEGF amount secreted from cells at different days of growth using ELISA.

Data is presented as mean±s.e.m. One-way ANOVA is performed for statistical analysis, and the data with statistically significant difference are labelled with different letters (a, b, c, d = p<0.05).