Table 4. Maximum number of samples per pool for each of the pooling methods for sample sizes N = 96, 384, and 1,536, when k = 1 and k = 20.

	No. of Samples	DNA Sudoku	2D Pooling	S-Stage	Halving	Gen. Binary Splitting	Modified 3-Stage
Max No. Samples per Pool (k = 1, $\widehat{k}=1$)	96	10	10	48	96	64	24
	384	20	20	192	384	256	55
	1,536	40	40	768	1,536	1,024	140
Max No. Samples per Pool (k = 20, $\widehat{k}=20$)	96	10	3	3	96	2	3
	384	20	4	8	384	16	8
	1,536	40	4	34	1,536	64	20

The best and worst performing methods in each row are indicated in green and orange, respectively.