Figure 2.

DNAM-1, TIGIT and CD96 are dose-dependently upregulated by rhIL-15 and DNAM-1 is downregulated by OC tumor cells. (a) DNAM-1 (n = 8), TIGIT (n = 6) and CD96 (n = 5) expression on NK cells of healthy donors co-cultured with SKOV-3, IGROV-1 or OVCAR-3 spheroids and increasing rhIL-15 concentrations for 7 days. Data is shown as mean+SEM. One-Way ANOVA with Bonferroni correction was used for statistical analysis, * p < .05, ** p < .01 and *** p < .001. (b) DNAM-1, TIGIT and CD96 expression on healthy donor NK cells co-cultured with a patient-derived primary tumor cell line and increasing rhIL-15 concentrations for 7 days cultured in duplicate. (c) DNAM-1, TIGIT and CD96 expression on healthy donor NK cells co-cultured with patient-derived tumor cells and increasing rhIL-15 concentrations for 7 days cultured in duplicate