FIG 2.

Early increase in intermediate monocytes after SARS-CoV-2 infection. (A) Gating strategy for the identification of different monocyte subsets, classical monocytes (CM), intermediate monocytes (IM), and nonclassical monocytes (NCM), by flow cytometry after gating out dead cells and lineage (CD3, CD20) cells and gating on HLA-DR⁺ cells. Early time point flow cytometry counterplots from P1 to P5 as well as that of a healthy control (HC) are shown. (B) Percentages of CM, IM, and NCM calculated from the lymphocyte live gate for each patient at different days POS. The dotted line represents one healthy donor. (C) Geometric mean (GM) fluorescent intensity (MFI) values for different activation and migration markers in intermediate monocytes. The dotted line in red shows the mean of the values for the isotype controls for each marker.