FIG 2.

RgdA is required for proper fungal growth and development. (A) Colonies of WT (AF293), ΔrgdA, and complemented (C′) strains point-inoculated on solid MMY and grown for 4 days. (B) Numbers of conidia produced by each strain per growth area. (C) mRNA levels of the key asexual developmental regulators in the ΔrgdA mutant relative to the WT at 24 h determined by qRT-PCR. Fungal cultures were done in liquid MMY, and mRNA levels were normalized using the ef1α gene. Data are means and standard deviations from three independent experiments. **, P < 0.01 (analysis of variance [ANOVA]).