FIG 4.

RgdA is necessary for proper spore germination. (A) Kinetics of germ tube outgrowth in A. fumigatus strains inoculated in liquid CM and MMG at 37°C The number of conidia showing germ tube protrusion was recorded at 2-h intervals and is presented as a percentage of the total number of conidia in each microscope field. CM, complete medium; MMG, glucose minimal medium; C′, complemented strain. (B) Germinated spores. Conidia were inoculated in MMG and incubated at 37°C for 14 h. (C) Effect of rgdA on conidial germination-regulatory genes. RNA was extracted from 24-h cultures of each strain. Strains were incubated in MMY medium at 37°C, and mRNA levels were normalized using the ef1α gene. Data are means and standard deviations from three independent experiments. **, P < 0.01 (ANOVA).