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. 2020 Nov 11;5(6):e00998-20. doi: 10.1128/mSphere.00998-20

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Copyright © 2020 Jun et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — RgdA downregulates GT production. (A) Heat map of genes encoding toxin-related proteins. Most gliotoxin biosynthetic genes were upregulated by the loss of rgdA. (B) qRT-PCR analysis of GT-related genes in WT, ΔrgdA, and complemented (C′) strains. Statistical differences between WT and mutant strains were evaluated with ANOVA. **, P < 0.01. (C) Determination of GT production in WT, ΔrgdA, and complemented strains. The culture supernatant of each strain was extracted with chloroform and subjected to TLC. The arrow indicates GT.