TABLE 2.

qPCR detection results for agricultural water samples seeded with Cyclospora cayetanensis oocysts

No. of seeded oocystsa	qPCR 18S target CT valuesb,d	No. of qPCR-positive replicatesb	IAC target CT value (mean ± SD)c
200	32.4 ± 0	3	29.2 ± 0.1
200	32.3 ± 0.5	3	28.1 ± 0.3
200	32.5 ± 0.1	3	27.8 ± 0.5
200	32.8 ± 0.2	3	28.4 ± 0.5
200	31.7 ± 0.2	3	27.7 ± 0.5
200	31.6 ± 0.2	3	27 ± 0.4
100	33.6 ± 0.2	3	32.1 ± 0.3
100	33.2 ± 0.1	3	32.3 ± 1.2
100	31.3 ± 0.1	3	28.1 ± 0.4
25	35.3 ± 0.3	3	26.9 ± 0.1
25	36.1 ± 0.7	3	26.1 ± 0.4
25	35.8 ± 0.3	3	26.4 ± 0.1
25	34.9 ± 0.4	3	28 ± 0.4
25	36.2 ± 0.2	3	28.9 ± 0.8
25	35.5 ± 0.7	3	26.6 ± 0.3
12	36.2 ± 0.3	3	29.1 ± 0.5
12	36.8 ± 0.6	3	29 ± 0.7
12	37.7 ± 2.7	3	29.2 ± 0.8
6	38.1 ± 0.4	3	30.4 ± 1.2
6	38.4 ± 0.6	2	28.7 ± 0.4
6	38.8	1	32.7 ± 1.5
6	36.8	1	28.7 ± 0.5
6	38.2 ± 0.8	2	34.3 ± 1.6
6	Undeterminede	0	31.3 ± 0.9
6	38.9	1	29.2 ± 0.2
6	Undetermined	0	29.4 ± 0.4
6	38.6	1	28.2 ± 0.3
6	38.1 ± 0.3	2	26.4 ± 0.4
6	Undetermined	0	24 ± 0.3
6	Undetermined	0	24.6 ± 1.6
0	Undetermined	0	27.7 ± 0.8
0	Undetermined	0	29.2 ± 0.6
0	Undetermined	0	27.1 ± 0.6
0	Undetermined	0	29.4 ± 0.3
0	Undetermined	0	27 ± 0.1
0	Undetermined	0	27.6 ± 0.3
0	Undetermined	0	26.7 ± 0.4
0	Undetermined	0	26 ± 0.1
0	Undetermined	0	27.4 ± 0.5

^aOocysts were seeded in 10-liter agricultural water samples as described in Materials and Methods.

^bNumber of qPCR positives out of the 3 replicates tested.

^cMean (±SD) C_T value for the C. cayetanensis 18S rRNA target reaction based on replicates producing a positive result (C_T ≤ 40.0). No SD is shown when only one replicate produced a positive result.

^dData represent mean values from positive replicates.

^eUndetermined, no C_T in qPCR.