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Summary of detection results using DEUF and qPCR for agricultural water samples seeded with C. cayetanensis oocysts or unseeded
| Matrix | Seeding levela | No. of samples analyzed | No. of oocysts/liter | qPCR |
|
|---|---|---|---|---|---|
| No. of positive samples | % positive | ||||
| Irrigation water (10 liters) | 0 | 12 | 0 | 0 | 0 |
| 6 | 12 | 0.6 | 8 | 66.6 | |
| 12 | 3 | 1.2 | 3 | 100 | |
| 25 | 6 | 2.5 | 6 | 100 | |
| 100 | 3 | 10 | 3 | 100 | |
| 200 | 6 | 20 | 6 | 100 | |
Oocysts were seeded in 10-liter agricultural water samples as described in Materials and Methods.
