TABLE 4.
qPCR results for detection of C. cayetanensis in DNA extracted from environmental samples processed by the DEUF method and qPCR
| Sample | Origina | Turbidity (NTU) | qPCR result | qPCR CTc |
|---|---|---|---|---|
| W33 | C&O Canal (Great Falls, MD) | 10.2 | Positive | 35.7 |
| W37 | C&O Canal (Great Falls, MD) | 17.3 | Negative | Undetermined |
| W40 | C&O Canal (Great Falls, MD) | 14.7 | Negative | Undetermined |
| W41 | C&O Canal (Great Falls, MD) | 14.9 | Negative | Undetermined |
| W42 | C&O Canal (Great Falls, MD) | 14.6 | Positive | 36.6 |
| W43 | C&O Canal (Great Falls, MD) | 16.5 | Positive | 33.9 ± 0.4 |
| Stool 28 controlb ,d | USA | NA | Positive | 29 ± 0.2 |
| Stool 19 controlb ,d | USA | NA | Positive | 27.1 ± 0.3 |
| Oocysts controlb ,e | Purified oocysts (Indonesia) | NA | Positive | 31 ± 0.3 |
a
A through F indicate different locations of the C&O Canal.
b
DNA extracted from clinical samples positive for C. cayetanensis and purified oocysts were included for comparison.
C
No SD is shown when only one replicate produced a positive result.
d
Stools 19 and 28 represent DNA from clinical samples from two individuals positive for C. cayetanensis by microscopic and molecular analysis.
e
Oocysts control represents DNA from oocysts purified from stool from an Indonesian patient.