TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant propertiesa | Source or reference |
|---|---|---|
| Strains | ||
| S. mutans | ||
| UA159 | Wild type ermS kanS specS | Wild type |
| YN102 | UA159 SMU_1405c::Pldh-ZsGreen ermBP | (56) |
| ΔcomDE | UA159 comDE deletion mutant; ermBP | This study |
| ΔcipB | UA159 cipB deletion mutant; ermBP | This study |
| ΔcomR | UA159 comR deletion mutant; ermBP | This study |
| ΔcomS | UA159 comS deletion mutant; ermBP | This study |
| ΔcomX | UA159 comX deletion mutant; ermBP | This study |
| ΔlytF | UA159 lytF deletion mutant; ermBP | This study |
| lytF comp.b | UA159 lytF deletion mutant; ermBP, SMU_437c::PlytF lytF aph3 | This study |
| PlytF reporter | UA159 SMU_437c::PlytF-mScarlet-I aph3 | This study |
| Pldh reporter | UA159 SMU_1405c::Pldh-mScarlet-I ermBP | This study |
| PcipB reporter | UA159 SMU_437c::PcipB-mScarlet-I aph3 | This study |
| Pldh and PlytF dual reporter | UA159 SMU_437c::PlytF-mScarlet-I aph3, SMU_1405c::Pldh-mNeonGreen ermBP | This study |
| ΔlytF Pldh reporter | UA159 lytF deletion mutant; aad9, SMU_1405c::Pldh-mScarlet-I ermBP | This study |
| lytF comp. Pldh reporter | UA159 lytF deletion mutant; aad9, SMU_437c::PlytF lytF aph3, SMU_1405c::Pldh-mScarlet-I ermBP | This study |
| ΔgtfB PlytF reporter | UA159 gtfB deletion mutant; ermBP, SMU_437c::PlytF-mScarlet-I aph3 | This study |
| ΔluxS PlytF reporter | UA159 luxS deletion mutant; ermBP, SMU_437c::PlytF-mScarlet-I aph3 | |
| B. subtilis | ||
| TAY3203 | trpC2 lys1 ΔaprE3 nprE18 nprR2 ΔydiR-ydjA ΔyqpP-yodU (ΔSPβ) amyE::nonA-spoVG 3′ UTR aph3 | (58) |
| Plasmids | ||
| pJIR418 | ermBP | (54) |
| pEX-A2J1-mScarlet-I | mScarlet-I | Eurofins genomics |
| pEX-A2J1-mNG | mNeonGreen | Eurofins genomics |
| pDL278 | aad9 | (55) |
a
ermS, erythromycin susceptibility; kanS, kanamycin susceptibility; specS, spectinomycin susceptibility.
b
comp., complemented.