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. 2020 Nov 10;86(23):e01359-20. doi: 10.1128/AEM.01359-20

FIG 2.

FIG 2

Growth properties of the host strain KU216 and the gene disruption strains. Growth of the host KU216 strain (circles) and the ΔTK2109 (squares), ΔTK2248 (triangles), and ΔTK2109 ΔTK2248 (diamonds) mutants was examined in a synthetic amino acid medium without serine and lipoic acid (mASW-AA-Ser[−]-S0-Ura-Lip[−]) (A), in a synthetic medium supplemented with lipoic acid (mASW-AA-Ser[−]-S0-Ura-Lip[+]) (B), and in a medium supplemented with octanoic acid (mASW-AA-Ser[−]-S0-Ura-Oct[+]) (D). (C) Growth of the host KU216 strain (circles) and ΔTK2109 ΔTK2248 mutant (diamonds) in a synthetic medium (mASW-AA-S0-Ura) with (open symbols) or without (closed symbols) biotin. Error bars indicate the standard deviations of three independent culture experiments. The vertical axis is represented in logarithmic scale.