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. 2020 Nov 10;86(23):e01359-20. doi: 10.1128/AEM.01359-20

FIG 4.

FIG 4

Lipoyl synthase activity measurement by HPLC analysis. HPLC analyses were performed with standard octanoyl-peptide (A), standard lipoyl-peptide (B), reduced lipoyl-peptide (C), reaction product without proteins (D), reaction product with reconstituted TK2109 protein (E), reaction product with reconstituted TK2248 protein (F), and reaction product with reconstituted TK2109 and TK2248 proteins (G). Abbreviations: OP, octanoyl-peptide; LP, lipoyl-peptide; RLP, reduced lipoyl-peptide; U1 to U4, unidentified compounds 1 to 4; AU, arbitrary units.