Table 1. Effect of BI01383298 on the uptake function of different transporters in HepG2 cells monitored with appropriate substrates and experimental conditions.

Substrate	Control	BI01383298	% Control
Citrate (7 µM)	483 ± 13	88.2 ± 5.6	18 ± 1**
Nicotinate (8 µM)	124 ± 3	123 ± 4	100 ± 3
Arginine (5 µM)	928 ± 31	935 ± 51	101 ± 5
Glutamine (5 µM)	319 ± 25	306 ± 3	92 ± 1
Tryptophan (5 µM)	80 ± 2	76 ± 3	95 ± 3
Lactate (3 µM)	141 ± 14	137 ± 7	97 ± 5

HepG2 cells were preincubated in NaCl buffer with or without 10 μM BI01383298 for 30 min. After washing the cells, uptakes of [¹⁴C]-citrate, [¹⁴C]-nicotinate, [³H]-arginine, [³H]-glutamine, [³H]-tryptophan, and [¹⁴C]-lactate were measured for a given time (which varied for different transporters) in NaCl buffer pH 7.5 (citrate, nicotinate, arginine), NMDG buffer pH 7.5 (glutamine, tryptophan) or NMDG pH 6 (lactate). The units for the uptake activity were pmol/mg of protein/30 min for citrate, pmol/mg of protein/15 min for nicotinate, arginine, glutamine, and tryptophan, and pmol/mg of protein/3 min for lactate. Data represent means ± S.D. ** P < 0.01 compared with the corresponding control uptake.