No changes in sulfur pools in 3-MST−/− mice. Myocardial H2S measured by methylene blue (A), H2Sn measured by SSP4 (B), sulfane sulfur (C) and free H2S measured by gas chromatography (D) in WT and 3-MST−/− mice. Serum H2S measured by methylene blue (E), sulfane sulfur (F) and free H2S measured by gas chromatography (G) in WT and 3-MST−/− mice. Values are shown as mean ± SEM, n = 6–9/group (A, B, E), n = 15/group (C, D, F, G).