Table 2.
Lethality, CiTX content and inhibition binding potency of digestive-gland extracts from mussels collected in Ingril lagoon that were contaminated with high levels of pinnatoxin-G.
| Extr | Surv. time (min) mean ± SEM; n | GYM-A (μg/kg) | 13SPX-C (μg/kg) | 20meSPX-G (μg/kg) | PnTX-A (μg/kg) | PnTX-G (μg/kg) | Inh. binding (%) mean ± SEM |
|---|---|---|---|---|---|---|---|
| DG-01 | 67.0 ± 0.7 ; 3 | 11.6 | 52.8 ± 3.8 | ||||
| DG-02 | 34.0 ± 0.7 ; 3 | 77.4 | 80.3 ± 0.1 | ||||
| DG-03 | 79.3 ±0.4 ; 3 | 52.1 | 83.1 ± 0.6 | ||||
| DG-04 | < 1440 ; 3 | 1.4 | 18.8 | 72.3 ± 3.2 | |||
| DG-05 | 70.5 ± 0.5 ; 2 | 2.1 | 65.3 ± 3.2 | ||||
| DG-06 | 12.0 ± 2.0 ; 3 | 40.3 | 73.6 ± 2.3 | ||||
| DG-07 | 4.0 ± 0.7 ; 3 | 1.8 | 1.3 | 37.2 | 78.9 ± 1.0 | ||
| DG-08 | 22.7 ± 2.9 ; 3 | 1.8 | 2.1 | 12.1 | 80.3 ± 1.4 | ||
| DG-09 | < 1440 ; 3 | 55.8 ± 0.9 | |||||
| DG-10 | 27.7 ± 2.2 ; 3 | 12.0 | 73.8 ± 0.9 | ||||
| DG-11 | < 1440 ; 3 | 2.8 | 3.1 | 96.4 ± 0.4 | |||
| DG-12 | 32.7 ± 3.6 ; 3 | 4.8 | 2.0 | 89.3 ± 0.6 | |||
| DG-13 | < 1440 ; 3 | 3.5 | 67.2 ± 2.4 | ||||
| DG-14 | 17.0 ± 0.7 ; 3 | 60.5 | 88.4 ± 0.2 | ||||
| DG-15 | < 1440 ; 3 | 10.6 | 2.2 | 24.7 ± 3.2 | |||
| DG-16 | 73.7 ± 0.4 ; 3 | 65.5 | 83.1 ± 0.7 |
The survival time is the mean value obtained after intraperitoneal injection of shellfish extracts on three mice (n). Abbreviations: GYM-A: gymnodimine-A; 13-SPX-C: 13-desmethyl spirolide-C; 20meSPX-G: 20-methyl spirolide-G; PnTX-A: pinnatoxin-A; PnTX-G: pinnatoxin-G. Toxin quantification was performed by UPLC-MS/MS in the MRM mode. Inhibition binding was performed by microplate-RBAssay. Inhibition binding percent are expressed as mean values of sextuplicate assays of at least two independent experiments.