Fig. 4.

Epitope mapping of the cRBD and complementarity-determining region (CDR) annotation of the mAbs. A) Anti-sRBD mAbs (single-chain variable fragments (scFv)) and their CDRs are shown. B) The variable light (VL) and variable heavy (VH) chains of the scFv regions of the reported anti-SARS-CoV-2 RBD mAbs are superimposed and the CDR regions are annotated according to Chothia and Lesk numbering scheme. C) The epitope prediction was validated through sRBD-F26G19 complex (PDB ID: 3BGF). The tabular interface is reported in the crystal structure while red boxes in the aligned sequences show the EpiPred predicted epitope. D) Conformational epitopes predicted with reference to six known anti-sRBD mAbs are highlighted and encircled. Residues participating in epitopes are indicated with arrows in the aligned cRBD and sRBD a.a. sequences (the arrow colors correspond to their respective epitopes). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)