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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Int Immunopharmacol. 2020 Aug 28;88:106922. doi: 10.1016/j.intimp.2020.106922

Figure 1. Metabolomic profiling of CD4+ T cell subsets reveals differences in tryptophan metabolic pathway.

Figure 1.

Naive CD4+CD25− T cells were collected or polarized in vitro for 3 days, split 1:2, and cultured with IL-2 alone for an additional 2 days to generate Th1, Th17, or Treg. T cell subset lysates from 3 biological replicate samples were extracted and analyzed using high-resolution LC-QE-MS for determination of cellular metabolites. Relative levels of tryptophan, serotonin (5-HT) and kynurenine are shown. Samples are shown as box plots representing 6 biological replicates per sample. * = p < .05 compared with naïve.