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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Int Immunopharmacol. 2020 Aug 28;88:106922. doi: 10.1016/j.intimp.2020.106922

Figure 3. CD4 T cells differentially express serotonin-related genes.

Figure 3.

Naive CD4+CD25− T cells were collected or polarized in vitro for 3 days, split 1:2, and cultured with IL-2 alone for an additional 2 days to generate Th1, Th17, or Treg. T cell subset lysates from 3 biological replicate samples were subjected to qPCR to assess gene expression. (A) Relative expression levels of Slc6a4 VMAT2 are shown. (B) Relative expression levels of 5-HTR1a, 5-HTR1b, 5-HTR2 and 5-HTR7 are shown. Samples are normalized to naive T cells and data are shown as mean ± SD and are representative of 3 independent experiments, n.d. = not detected. * = p < .05