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. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: Dev Cell. 2020 Sep 7;55(3):272–288.e5. doi: 10.1016/j.devcel.2020.08.006

Figure 1. The Rag GTPase inhibits TORC1 in GATOR2 mutant Drosophila and HeLa cells.

Figure 1.

(A) Co-depletions of ragA or ragC rescue the small ovary phenotype of seh1RNAi females. ragARNAi, ragCRNAi and seh1RNAi TRiP constructs were driven by the germline specific driver nanos-GAL4VP16. mchRNAi: mCherry-RNAi. Scale bar: 1 mm.

(B) Knockdowns of ragA or ragC increase pS6K levels in seh1RNAi ovaries, error bars in pS6K quantification graph represent standard deviation. Unpaired Student’s t-test was used to calculate p values. mchRNAi: mCherry-RNAi. **: p < 0.01.

(C) Immunoblot for P-S6K (T389) in WT and WDR24-KO HeLa cells treated with control (NT) or RAGA siRNAs. Error bars represent standard deviation.

(D) Immunoblot for P-S6K (T389) in WT and WDR24-KO HeLa cells treated with control (NT) or RAGC siRNAs.

(E) mTOR and LAMP1 immunostaining and colocalization analysis in WT and WDR24-KO HeLa cells treated with control (NT), RAGA or RAGC siRNA. Scale bar: 10 μm. The colocalization coefficient was calculated based on 30 cells per condition. Error bars represent standard deviation. See also Figure S1.