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. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: Dev Cell. 2020 Sep 7;55(3):272–288.e5. doi: 10.1016/j.devcel.2020.08.006

Figure 5. TSC2 rapidly cycles on and off lysosomes in GATOR2 mutant and starved cells.

Figure 5.

(A) Time point images from photoconversion experiment in WT Hela cells transfected with mEOS4b-TSC2 in nutrient replete conditions. The 0s image represents photoconversion. Scale bar: 0.5 μm.

(B) Time point images from photoconversion experiment in WT Hela cells transfected with mEOS4b-TSC2 under starvation condition.

(C) Time point images from photoconversion experiment in WDR24-KO Hela cells transfected with mEOS4b-TSC2 subjected to nutrient replete conditions.

(D) Time point images from photoconversion experiment in WDR24-KO Hela cells transfected with mEOS4b-TSC2 under starvation condition.

(E-H) Change in relative fluorescence of unconverted mEOS4b-TSC2 at 488 nm (green curve) and photo converted protein in 568 nm (red curve) over 600s period corresponding to the results shown in A-D. A total of 30 lysosomes in each condition were used. Error bars represent standard error.

(I) Comparisons of the half-time (t1/2) for mEOS4b-TSC2 at 488 nm and 568 nm in corresponding to panels A-D. Scale bars represent standard error.