Skip to main content
. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: Dev Cell. 2020 Sep 7;55(3):272–288.e5. doi: 10.1016/j.devcel.2020.08.006

Figure 6. The Rag GTPase controls the dynamic recruitment of TSC to lysosomes in response to both amino acids and growth factor restriction.

Figure 6.

(A) Time point images from the Halo-TSC2 FRAP experiment in WT HeLa cells treated with control or RAGA siRNA in FED condition (+AA, +FBS). Scale bar: 0.5 μm. Fluorescence recovery versus time curve was plotted by using a total of 30 lysosomes in cells. Error bars represent standard error.

(B) Time point images from the Halo-TSC2 FRAP experiment in WDR24-KO HeLa cells treated with control or RAGA siRNA in FED condition (+AA, +FBS).

(C) Time point images from the Halo-TSC2 FRAP experiment in WT HeLa cells treated with control or RAGA siRNA in amino acid starvation (-AA, +dFBS).

(D) Time point images from the Halo-TSC2 FRAP experiment in WDR24-KO HeLa cells treated with control or RAGA siRNA in amino acid starvation (-AA, +dFBS).

(E) Time point images from the Halo-TSC2 FRAP experiment in WT HeLa cells treated with control or RAGA siRNA in serum starvation (+AA, -FBS).

(F) Time point images from the Halo-TSC2 FRAP experiment in WDR24-KO HeLa cells treated with control or RAGA siRNA in serum starvation (+AA, -FBS).