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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Nucl Med Biol. 2020 May 12;92:171–183. doi: 10.1016/j.nucmedbio.2020.05.002

Scheme 2.

Scheme 2.

Radiolabeling of the Boc2-MEGMTB tin precursor and the radioconjugation of [131I]MEGMIB to the anti-Her2 sdAb 5F7GGC. a) i: [131I]NaI, NCS, acetic acid, MeOH, ii: RP-HPLC; b) i: TFA, ii: 0.2 M NH4OAc, 5 mM EDTA buffer, 37 °C, 45 min; c) immobilized TCEP gel, 0.2 M NH4OAc, 5 mM EDTA buffer, 37 °C, 1 h.