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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Arch Toxicol. 2020 Aug 20;94(12):4007–4022. doi: 10.1007/s00204-020-02879-z

Fig. 4. Muscle-specific CncC signaling modulation of MeHg effects on eclosion ability and DIOM morphology.

Fig. 4

a, b Effect of muscle-specific CncC overexpression (Mef2>CncC) and knockdown (Mef2>CncCRNAi) on eclosion. c, d Effect of Keap1 knockdown (>Keap1RNAi) and overexpression (>Keap1) on eclosion compared to control (Mef2>w1118) (a-d n = 3, 450 flies/genotype/treatment, mean ± s.d.m., Z-test, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). e Representative images of MeHg effect on DIOM morphology without (>w1118 control) or with knockdown (>Keap1RNAi) or overexpression (>Keap1) of Keap1. Number of myospheres originating from the most medial DIOMs in the A2-A5 abdominal segments (indicated by white asterisks) were quantified: (f) 0 μM, (g) 5 μM, and (h) 10 μM MeHg (f-h n ≥ 20 abdomens/genotype/treatment, mean ± s.d.m., Kruskal-Wallis test with Dunn’s post-hoc for multiple comparisons, p<0.05, significant differences indicated by different letter labels).