Figure 2.

Relative frequencies of cTfh and their subsets in PIRD patients. The frequency of CD4⁺CD45RA^-CXCR5⁺ cTfh cells in a cohort of 24 HD and 14 patients with dysregulatory syndrome PID (A) and their distribution in each group of this cohort (B) were depicted. Also, the frequencies of CCR6^-CXCR3⁺ (cTfh1, C) and CCR6⁺CXCR3^- (cTfh17, D) cells within cTfh cells in this cohort, and the distribution of the frequencies of CCR6^-CXCR3⁺ (cTfh1, E) and CCR6⁺CXCR3^- (cTfh17, F) cells in each group of this cohort were depicted. In addition, the cTfh1/cTfh17 ratio (G) and its distribution in each group of this cohort (H) are shown. Horizontal lines in (A, C, D) represent the mean of each group. P1 (STAT1 GOF) and P11 (CVID) were under treatment (as detailed in Supplementary Table 1). Gray areas in (B, E, F) indicate the 10^th and 90^th percentiles of HD for each parameter. An unpaired parametric t-Student test with Welch´s correction was used in (A, C, D); a non-parametric test with Dunn’s post hoc was used in (B, E, F). An unpaired non-parametric t-Student test with Mann-Whitney U test was used in G. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.