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. Author manuscript; available in PMC: 2021 Nov 15.
Published in final edited form as: J Immunol. 2020 Oct 5;205(10):2679–2693. doi: 10.4049/jimmunol.2000434

Figure 4.

Figure 4.

Co-culture of stage 4A cells with OP9-DL1 stroma promotes the transition to a stage 4B-like phenotype in vitro. Representative (n ≥ 4 for each marker) flow cytometry analyses depicting relative expression of selected surface and intracellular markers comparing ILC3/stage 3-like (CD94−NKp80−, dotted black histograms), stage 4A-like (CD94+NKp80−, solid black histograms) and stage 4B-like (CD94+NKp80+, red histograms) cells generated in vitro following 14 day culture of freshly purified tonsil-derived stage 4A cells with IL-7 and OP9-DL1 cells. Analysis of IFN-γ by flow cytometry was measured following post-culture incubation for 4 hr with IL-12, IL-15, and IL-18 (10 ng/ml each). Analysis of IL-22 by flow cytometry was measured following post-culture incubation for 4 hr with IL-2, IL-1β, and IL-23 (10 ng/ml each).