Table 3.

Definitive screening design and experimental results

Conditions	Temperature (°C)	pH	DO (%)	Phosphate content (mM)	OD48 (600 nm)	DCW48 (g/L)	WCW48 (%)	Titer48 (g/L)
1	27	6.5	30	86.4	144	65.6	17.8	0.930
2	33	6.5	40	86.4	193	63.3	34.6	2.450
3	33	7.1	40	40.1	161	51.4	26.45	1.691
4	30	7.1	40	86.4	256	77	35.85	2.335
5	33	6.5	35	40.1	125.4	50.9	25.9	1.363
6	27	6.8	40	40.1	128	51.9	25.1	1.191
7	27	7.1	30	40.1	135.8	55	26.75	1.275
8	27	7.1	35	86.4	157.6	69.5	36.45	1.670
9	27	6.5	40	58.9	169.2	69.4	32.9	1.216
10	30	6.8	35	58.9	149.8	63.8	31.3	2.084
11	30	6.5	30	40.1	123.2	51.9	27.5	1.272
12	33	6.8	30	86.4	181	67.3	32.45	2.404
13	33	7.1	30	58.9	206	66.5	32.05	2.695
14	30	6.8	35	58.9	198	65.3	34.9	2.116
15	30	6.8	35	58.9	196	67.3	32.2	1.868
16	30	6.8	35	58.9	192	65.8	30.9	2.138