Fig. 6.

Pre-treating neurons with Aβ42 oligomers inhibits uptake of ovalbumin. Cells were treated either with 10 μM Aβ42 oligomers or equivalent volume of buffer for 72 h, followed by addition of 25 ug/mL Ova-Cy (a) or Ova-488 (d). Quantification of mean fluorescence intensity from Ova-Cy (b) or Ova-488 (e) in the cell body (one 0.5 μm slice) over 150 min from cells either treated with buffer (blue) or pre-treated with 10 μM Aβ42 oligomers for 72 h (red). Each trace arises from the same individual cell measured over time. Data pooled from three separate experiments 4–9 FOV per experiment, n (cells) buffer = 84, Aβ42 = 19 (b), 4–5 FOV per experiment, n (cells) buffer = 53, Aβ42 = 33 (e). Example images of live cells treated with Aβ42 oligomers (top) or equivalent volume of buffer (bottom) followed by Ova-Cy (c) or Ova-488 (f), taken at increasing time points. Fluorescence intensity is only reduced in cells treated with oligomers. Arrows point to neuronal cell bodies. One 0.5-μm slice is shown. Scale bar is 25 μm