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Primers used in this study
| Primer | Sequence (5′→3′) | Description |
|---|---|---|
| PRO1-0 | CAG TGA AGT GTT CAA GGG | For gene cloning |
| PRO1-5 | CTT CCA AGG GTA GGA AA | |
| PRO1_pET_fw | CGC GCG GCA GCC ATA TGA AGG ATG CTA ATG AGA G | |
| PRO1_pET_rv | CAG CCG GAT CCT CGA GTC AAC GAG GTG GGA ATG C | |
| PRO1_I150T_fw | GTT AGA GAA AC*C AAA TTT GGT GAC AAT GAC | For site-directed mutagenesis |
| PRO1_I150T_rv | ACC AAA TTT GG*T TTC TCT AAC AGA TAG TGT |
The asterisks indicate the positions of the nucleotide mutation
