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. 2020 Nov 11;10:19584. doi: 10.1038/s41598-020-76774-z

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of OCT RBC flux estimation. (a) 2D histogram displaying the estimation of RBC flux in both modalities, highlighting a concentration of measurements on the diagonal. (b) Absolute error between both modalities, binned into steps of 5 RBC/s. (c) Distribution of the differences in estimation for all measurements, with a mean of 2.5 RBC/s and variance of 10.7 RBC/s. (d) Marginal histograms of OCT and TPEF RBC flux estimations, highlighting a bias in OCT RBC flux towards the 20–60 RBC/s band. (e) Band analysis displaying the difference distributions for 5 bands of RBC flux: 0–20, 20–40, 40–60, 80–120 RBC/s.