FIGURE 1.

Isolation of patient-derived AUB-PrC cells using organoids culture conditions. Representative bright-field images showing established prostate organoids (generation 1, G1) from unaffected and tumor prostate patient samples {patient 2 with Grade Group 3 [Gleason Score 7(4 + 3)]; patient characteristics in Supplementary Table S1} grown in culture (Scale bar = 200 μm) (A), and AUB-PrC cells established and grown on 1% collagen type-I coated plates (Scale bar = 200 μm) (B) maintaining their healthy morphology when propagated for continuous passages (passages P8, P11, P15, P18, P22, and P28 are shown) reaching more than 28 passages with successful repeated freeze-thaw cycles (Scale bar = 100 μm) (C).