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. 2020 Oct 29;11:571372. doi: 10.3389/fphys.2020.571372

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Copyright © 2020 Musci, Walsh, Konopka, Wolff, Peelor, Reiser, Santangelo and Hamilton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Collagen content in the guinea pig skeletal muscle. As assessed with Masson’s Trichrome staining, there was a significant age-related decrease in collagen content in the gastrocnemius (A), but not the soleus (B). Representative images of stained 5 and 15 mo gastrocnemius cross sections (C). Assessed with spectrophotometry, collagen content was significantly lower in both the gastrocnemius (D) and soleus (E) of 15 mo Dunkin Hartley guinea pigs compared to 5 mo guinea pigs (p < 0.05). Scale bar (bottom left) = 50 μm. *denotes p < 0.05 compared to 5 mo.