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. 2020 Nov 11;11(11):965. doi: 10.1038/s41419-020-03183-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Representative images of spindle/chromosome organization in oocytes from aged mice with or without 10 μM quercetin treatment. Spindles were stained with α‐tubulin antibody (green), and chromosomes were counterstained with DAPI (blue). Scale bar, 10 μm. B Distinct cortical granules (CGs) distributions with or without quercetin treatment. CGs in oocytes were stained with lens culinaris (LCA)‐FITC (fluorescein isothiocyanate: green), and chromosomes were stained with DAPI (blue). Scale bar, 25 μm. C Quantification of abnormal spindle/chromosomes oocytes with or without quercetin treatment. D The proportion of oocytes with abnormally distributed CGs, following quercetin treatment. Data are means ± SD of at least three independent experiments. *P < 0.05 vs. control as calculated by two-tailed unpaired Student’s t-tests.