Fig. 1. Deletion of Ring1 in neural tissue reduces the amount of H2AK119ub and results in morphological defects in the early stage telencephalon.

a, c Coronal sections of the brain of control (Ring1b^flox/flox or Ring1b^flox/+) and Ring1B KO (Ring1b^flox/flox;Sox1-Cre) mice (a) or of Ring1A KO (Ring1a^–/–;Ring1b^flox/flox or Ring1a^–/–;Ring1b^flox/+) and Ring1A/B dKO (Ring1a^–/–;Ring1b^flox/flox;Sox1-Cre) mice (c) at E10 were subjected to immunohistofluorescence staining with antibodies to H2AK119ub. Nuclei were counterstained with Hoechst 33342. Scale bars, 200 μm. b, d The ratio of the average immunostaining intensity of H2AK119ub for the entire telencephalon to that for nonneural tissue adjacent to the ventral telencephalon was determined as relative intensity (A.U., arbitrary units) for images similar to those in a and c, respectively. Data are means ± s.d., n = 3 embryos of each genotype, two-tailed Student’s unpaired t test. e, g Images of the brain of control and Ring1B KO mice (e) or of Ring1A KO and Ring1A/B dKO mice (g) at E10 and E11. Scale bars, 1 mm. f, h Quantification of the lateral projected area of the telencephalon at E10 and E11 for images similar to those in (e) and (g). Data are means ± s.d., averaged values for n = 4 litters (the number and individual values of each littermate are provided in Source Data File), two-tailed Student’s paired t test. Source data are provided as a Source Data file.