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. 2020 Nov 11;11:5709. doi: 10.1038/s41467-020-19556-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Cells from the telencephalon (outside of the dorsal midline) of control or Ring1B KO mice at E10 were maintained in monolayer culture for 6 h and then exposed for 24 h to 2 μM SAG or dimethyl sulfoxide vehicle. b RT-qPCR analysis of relative Gli1 and Ptch1 mRNA abundance (normalized by the amount of Actb mRNA) in NPCs treated as in (a). Data are means ± s.d., n = 3 independent experiments, one-way ANOVA followed by Dunnett’s multiple-comparison test. c, e The telencephalon of WT (ICR) mice at E9 was maintained in explant (c) or monolayer (e) culture for 6 h before exposure for an additional 24 h to BMP4 (50 ng/ml) or 5 μM CHIR-99021. d, f RT-qPCR analysis of relative Shh mRNA abundance (normalized by the amount of Actb mRNA) in NPCs treated as in (c) and (e), respectively. Data are means ± s.d., n = 4 independent experiments, one-way ANOVA followed by Dunnett’s multiple-comparison test. g The telencephalon of WT (ICR) mice at E9 was maintained in monolayer culture for 6 h before exposure for an additional 24 h to 1 μM cyclopamine or ethanol (EtOH) vehicle. h, i RT-qPCR analysis of relative Bmp4, Bmp7, Wnt7b, or Wnt8b (h) or Gli1, Id1, or Axin2 (i) mRNA abundance (normalized by the amount of Actb mRNA) in NPCs treated as in (g). Data are means ± s.d., n = 3 independent experiments, two-tailed Student’s paired t test. Source data are provided as a Source Data file.