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. 2020 Nov 11;11:5709. doi: 10.1038/s41467-020-19556-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a The telencephalon was isolated from WT (ICR) mice at E9 for ChIP-qPCR analysis with antibodies to H3K27me3 or to Ring1B. b, c ChIP-qPCR analysis of H3K27me3 deposition (b) and Ring1B binding (c) at the indicated promoters as in (a). Hoxa1 and Hoxd3 were examined as positive controls, and Actb and Gapdh as negative controls. Data are expressed as percentage input and are means ± s.d., n = 3 independent experiments, two-tailed Student’s unpaired t test versus the corresponding value for Actb. Source data are provided as a Source Data file.