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. 2020 Nov 11;11:5709. doi: 10.1038/s41467-020-19556-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a CD133⁺ NPCs isolated from the dorsal midline (DM), CTX, and ventral (V) regions of the telencephalon from WT (ICR) embryos at E11 were subjected to CUT&Tag analysis with antibodies to H3K27me3 or to Ring1B. Biological triplicates (for H3K27me3) or quadruplicates (for Ring1B) whose quality of regional dissection was confirmed by RT-qPCR analysis of region-specific gene expression (Supplementary Fig. 8a) were analyzed. b, c Averages of normalized CUT&Tag signals around Bmp4 (b) or Wnt8b (c) in three (H3K27me3) or four (Ring1B) independent experiments are visualized in the UCSC genome browser. The RefSeq gene models are shown at the bottom of each panel. d, e Averages of normalized CUT&Tag signals for H3K27me3 around (±1 kbp) the transcription start site (TSS) (d) and Ring1B around (±1 kbp) the Ring1B summit (determined as the site in the gene body with the highest Ring1B signals across all samples) (e) of the indicated genes. Data are means ± s.d. for biological triplicates (d) or quadruplicates (e), one-way ANOVA followed by the Benjamini–Hochberg multiple-comparison test. Source data are provided as a Source Data file.