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NETs elicit macrophage production of Rantes and MIP-1β. HIV-1-infected macrophages were left untreated (medium) or treated with NETs (40 ng/mL) for 3 h, washed and maintained in culture during the indicated times. Supernatants were then collected, and the concentrations of Rantes (a) and MIP-1 β (b) were measured by ELISA. Each point represents a donor. Data represent means ± SD, which were analyzed using Kruskal–Wallis test with Dunn post-test. *p < 0.05; **p < 0.01.
